kato iii cells Search Results


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Procell Inc kato-iii cell line
GC cells can stimulate neutrophils to form NETs. A and B: Control neutrophils were cocultured with normal gastric mucosal epithelial cells (GES-1) or GC cells (AGS, MKN-45 and <t>KATO-III)</t> and NET formation was measured by confocal microscopy with cell-impermeable Sytox-Green staining. Magnification 20×; scale bars: 50 μm. Green: Neutrophils; C and D: Control neutrophils were cocultured with CM from GES-1 or GC cells, and stained with MPO and citH3. The percentage of NET-releasing cells was defined as the ratio of the calculated NET releasing neutrophils to the total number of neutrophils. Magnification 20×; scale bars: 50 μm. Red-citH3, Green-MPO, and Blue-DAPI. All values are the mean ± SD. a P < 0.05; b P < 0.01; c P < 0.001; d P < 0.0001. GC: Gastric cancer; GES-1: Gastric mucosal epithelial cells; NET: Neutrophil extracellular trap; CM: Conditioned medium; MPO: Myeloperoxidase; citH3: Citrullinated histone H3.
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Becton Dickinson kato iii/vcr cells
GC cells can stimulate neutrophils to form NETs. A and B: Control neutrophils were cocultured with normal gastric mucosal epithelial cells (GES-1) or GC cells (AGS, MKN-45 and <t>KATO-III)</t> and NET formation was measured by confocal microscopy with cell-impermeable Sytox-Green staining. Magnification 20×; scale bars: 50 μm. Green: Neutrophils; C and D: Control neutrophils were cocultured with CM from GES-1 or GC cells, and stained with MPO and citH3. The percentage of NET-releasing cells was defined as the ratio of the calculated NET releasing neutrophils to the total number of neutrophils. Magnification 20×; scale bars: 50 μm. Red-citH3, Green-MPO, and Blue-DAPI. All values are the mean ± SD. a P < 0.05; b P < 0.01; c P < 0.001; d P < 0.0001. GC: Gastric cancer; GES-1: Gastric mucosal epithelial cells; NET: Neutrophil extracellular trap; CM: Conditioned medium; MPO: Myeloperoxidase; citH3: Citrullinated histone H3.
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Verlag GmbH cancer cell kato-iii
GC cells can stimulate neutrophils to form NETs. A and B: Control neutrophils were cocultured with normal gastric mucosal epithelial cells (GES-1) or GC cells (AGS, MKN-45 and <t>KATO-III)</t> and NET formation was measured by confocal microscopy with cell-impermeable Sytox-Green staining. Magnification 20×; scale bars: 50 μm. Green: Neutrophils; C and D: Control neutrophils were cocultured with CM from GES-1 or GC cells, and stained with MPO and citH3. The percentage of NET-releasing cells was defined as the ratio of the calculated NET releasing neutrophils to the total number of neutrophils. Magnification 20×; scale bars: 50 μm. Red-citH3, Green-MPO, and Blue-DAPI. All values are the mean ± SD. a P < 0.05; b P < 0.01; c P < 0.001; d P < 0.0001. GC: Gastric cancer; GES-1: Gastric mucosal epithelial cells; NET: Neutrophil extracellular trap; CM: Conditioned medium; MPO: Myeloperoxidase; citH3: Citrullinated histone H3.
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Image Search Results


GC cells can stimulate neutrophils to form NETs. A and B: Control neutrophils were cocultured with normal gastric mucosal epithelial cells (GES-1) or GC cells (AGS, MKN-45 and KATO-III) and NET formation was measured by confocal microscopy with cell-impermeable Sytox-Green staining. Magnification 20×; scale bars: 50 μm. Green: Neutrophils; C and D: Control neutrophils were cocultured with CM from GES-1 or GC cells, and stained with MPO and citH3. The percentage of NET-releasing cells was defined as the ratio of the calculated NET releasing neutrophils to the total number of neutrophils. Magnification 20×; scale bars: 50 μm. Red-citH3, Green-MPO, and Blue-DAPI. All values are the mean ± SD. a P < 0.05; b P < 0.01; c P < 0.001; d P < 0.0001. GC: Gastric cancer; GES-1: Gastric mucosal epithelial cells; NET: Neutrophil extracellular trap; CM: Conditioned medium; MPO: Myeloperoxidase; citH3: Citrullinated histone H3.

Journal: World Journal of Gastroenterology

Article Title: Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer

doi: 10.3748/wjg.v28.i26.3132

Figure Lengend Snippet: GC cells can stimulate neutrophils to form NETs. A and B: Control neutrophils were cocultured with normal gastric mucosal epithelial cells (GES-1) or GC cells (AGS, MKN-45 and KATO-III) and NET formation was measured by confocal microscopy with cell-impermeable Sytox-Green staining. Magnification 20×; scale bars: 50 μm. Green: Neutrophils; C and D: Control neutrophils were cocultured with CM from GES-1 or GC cells, and stained with MPO and citH3. The percentage of NET-releasing cells was defined as the ratio of the calculated NET releasing neutrophils to the total number of neutrophils. Magnification 20×; scale bars: 50 μm. Red-citH3, Green-MPO, and Blue-DAPI. All values are the mean ± SD. a P < 0.05; b P < 0.01; c P < 0.001; d P < 0.0001. GC: Gastric cancer; GES-1: Gastric mucosal epithelial cells; NET: Neutrophil extracellular trap; CM: Conditioned medium; MPO: Myeloperoxidase; citH3: Citrullinated histone H3.

Article Snippet: The human metastatic GC KATO-III and MKN-45 cell lines, human primary GC AGS cell line, human gastric mucosal epithelial cell (GES-1) line, human umbilical cord endothelial cell (HUVEC) line, and mouse forestomach squamous carcinoma (MFC) cell line were purchased from PROCELL (Wuhan, China).

Techniques: Confocal Microscopy, Staining