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Image Search Results
Journal: Journal of inorganic biochemistry
Article Title: Chirality influence on the cytotoxic properties of anionic chiral bis(N-heterocyclic carbene)silver complexes.
doi: 10.1016/j.jinorgbio.2022.111924
Figure Lengend Snippet: Fig. 3. Effect of complexes 2b/2b’ and 2c/2c’ on the viability of human non-malignant cells (HaCaT) and human cancer cells (A549, MeWo, T24 and KATO III). Cells were exposed to several concentrations of compounds for 72 h and cell viability was measured using the resazurin assay. Data represent mean ± SEM from at least two independent experiments.
Article Snippet: HaCaT cells (human keratinocytes [51]), A549 (human non-small cell lung cancer cells),
Techniques: Resazurin Assay
Journal: The Kaohsiung Journal of Medical Sciences
Article Title: GLIS2 Promotes Epithelial‐Mesenchymal Transition and Gastric Cancer Progression by Regulating BGN to Activate the Wnt/β‐Catenin Pathway
doi: 10.1002/kjm2.70103
Figure Lengend Snippet: GLIS2 expression was increased in GC and related to poor prognosis. (A) The UALAN database was used to predict GLIS2 expression in GCs. (B) The association of GLIS2 with prognosis was analyzed by the Kaplan–Meier method. Eighteen pairs of cancerous and paraneoplastic tissues from patients with GC were collected for experimental analysis. (C) The mRNA expression of GLIS2 was detected using RT‐qPCR. N = 18. Statistical significance was determined by Student's t‐test: *** p < 0.001. (D) The levels of GLIS2 were examined by western blotting. N = 4. Statistical significance was determined by Student's t‐test: ** p < 0.01. RNA and protein were collected from normal gastric epithelial cells (GES‐1) and GC cell lines (MKN74, MKN1, KATO‐III, AGS, and SNU‐16), respectively. (E) RT‐qPCR was performed to detect the GLIS2 expression. (F) Western blotting was employed to analyze the protein level of GLIS2. The experiment was performed in triplicate. Statistical significance was determined by Student's t‐test: * p < 0.05, ** p < 0.01, and *** p < 0.001.
Article Snippet:
Techniques: Expressing, Quantitative RT-PCR, Western Blot